Quantitation by GC and GC-MS

Quantitation by GC and GC-MS

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Quantitation by GC and GC-MS, takes an in depth look at some of the more common quantitation protocols, explains their use and how to successfully put them into practice. The aim of the course is to give students an advanced understanding of the requirements for accurate quantitation, how to choose between approaches, how to use them correctly, how to ensure the developed methods successfully solve lab problems, and how to better understand and interpret results. The course is aimed at more experienced users, who wish to extend their skills and understanding, and at laboratory managers who want a better knowledge of the techniques they supervise. The course covers: Necessary method development for accurate quantitation using GC and GC-MS; A logical and systematic explanation of the different quantitation methods (internal normalization, external standard, internal standard, standard addition) and the pitfalls which may be encountered using them; Response factors and relative response factors; Isotope dilution techniques, the most efficient and accurate method for low level quantification using MS; Interpretation and reporting of results.