Date: 23 October - 3 November 2023
Duration: 2 weeks
Venue: NMISA Training Center, Building 4, CSIR Campus, Pretoria
Course Objectives:
Supporting sustainable agricultural practices, ecosystem health and exports through accurate measurement.
Liquid chromatography (LC) is becoming an increasingly popular separation method in organic analysis. New generation synthetic compounds, including many pesticides and pharmaceuticals are larger, less volatile, and more polar, making LC the preferred separation technique. Additionally, a large variety of detectors can be used in combination with LC separation.
Therefore, LC is a highly versatile technique that can be applied in a multitude of fields. This versatility, in part, can be attributed to the variety of column phases, dimensions, physical attributes such as pore size and dimensions. After the selection of a column separation can further be influenced by the mobile phase. This includes solvent strengths, pH and the use of buffers and modifiers. The number of choices during method development can be very daunting to analysts.
The aim of this course is to provide analysts with tools to improve the separation, identification, and quantitation of compounds of interest by liquid chromatograph